Figure 4. IL-17-producing cells are more frequent in brain abscesses of TLR2 KO mice.

T cells were recovered from brain abscesses, draining cervical lymph nodes (CLN), and spleens of TLR2 KO and WT mice at days 7 and 14 following S. aureus exposure using CD90-conjugated magnetic beads and stimulated with a cocktail of PMA + ionomycin for 12 h in the presence of GolgiBlock^™, whereupon IL-17 and IFN-γ production was quantitated by intracellular cytokine staining and FACS. The results presented are representative of three independent experiments.