Figure 5. γδ T cells are a source of IL-17 in brain abscesses.
Cells were recovered from brain abscesses of C57BL/6 wild type mice at day 14 following S. aureus exposure using CD90-conjugated magnetic beads and stimulated with a cocktail of PMA + ionomycin for 12 h in the presence of GolgiBlock™, whereupon IL-17 production in CD4+ Th17 or γδ T cells was quantitated by intracellular cytokine staining. The results presented are representative of two independent experiments.