Figure 8. IL-17R isoform expression in astrocytes.
TLR2 KO and WT astrocytes were seeded at 1 × 106 cells per well in 6-well plates and incubated overnight. The following day, cells were stimulated with 107 heat-inactivated S. aureus for 6, 12, or 24 h, whereupon total RNA was isolated and examined for IL-17RA (A) and IL-17RC (B) expression by qRT-PCR. Gene expression levels were calculated after normalizing IL-17R signals against GAPDH and are presented in relative mRNA expression units (mean ± SEM of two independent experiments).