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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Glia. 2009 Sep;57(12):1316–1325. doi: 10.1002/glia.20851

Figure 3. MMP-9 activates ERK1/2 and suppresses Schwann cell growth.

Figure 3

A, MMP-9 activates ERK1/2 in a dose-dependent manner. 100 nM rhMMP-9 was selected for use in the subsequent experiments. B, A time-course of rhMMP-9 treatment demonstrating a biphasic activation of ERK1/2. C, Immunofluorescence for pERK (green) and DAPI (blue) confirms the biphasic reactivity of cytosolic pERK at 15 min and 6 h, objective magnification × 100 (representative micrographs of 3 independent experiments). D, Schwann cell growth curve studies using crystal violet, with and without daily rhMMP-9 stimulation for 72 h. Data represents the mean ± SD of N=3/group, analyzed by one-way ANOVA (*, p<0.05).