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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Glia. 2009 Sep;57(12):1265–1279. doi: 10.1002/glia.20846

Figure 3. The binding and regulatory ability of predicted PMP22 targeting miRNAs.

Figure 3

(A) MiRNAs that are predicted to bind to the 3′UTR of PMP22 are shown. (B) Binding of candidate miRNAs are detected as miRNA/PMP22 RNA complexes by a gel shift assay. The RNA alone lane contains PMP22 RNA only, whereas miR-124 serves as an additional negative control. Total RNA input is shown using SYBR gold staining. (C) Luciferase assays were performed after co-transfection of the PMP22 3′UTR luciferase reporter and the indicated miRNA-precursors. Luciferase signal is shown after Renilla Luciferase (RL) readings were normalized to Firefly Luciferase (FL). Neg. miR does not code any known miRNA, and miR-124 is used as a non-PMP22 targeting control. (*p<0.05, n=3), n.s.: not significant.