Figure 4. Growth conditions alter the miRNA expression profile of Schwann cells.
(A) The miRNA expression profiles of Schwann cells grown in serum-containing (proliferating) or defined medium (non-proliferating) were determined using an Exiqon microarray (n=3 for each condition, color scale bar indicates fold-change). (B) Three miRNAs predicted to target the 3′UTR of PMP22 are expressed in Schwann cells and show unique profiles upon growth condition. (C) The fold-change in the expression of predicted PMP22 regulating miRNAs in proliferating, as compared to non-proliferating Schwann cells (*p<0.05, n=3, 2−ΔΔCT method). MiR-124a serves as a negative control.