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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Glia. 2009 Sep;57(12):1265–1279. doi: 10.1002/glia.20846

Figure 5. miR-29a regulates PMP22 3′UTR-luciferase reporter expression through one specific binding site.

Figure 5

(A) Schwann cells were co-transfected with the 3′UTR luciferase reporter and either miR-29a or anti-miR-29a, and luciferase activity was quantified (**p<0.01, n=4). (B) Inhibition of miR-322/424, miR-381, and miR-140* does not significantly increase PMP22 3′UTR reporter expression when compared to Neg. control (n=3, p>0.05). (C) Schematic representation of PMP22 3′UTR truncation constructs. The predicted miR-29a binding site is at 0.66 kb after the stop codon (grey line). (D) Quantification of luciferase assays after transfection with the indicated constructs (**p<0.01; *p<0.05; ***p<0.001, n=4). (E) A schematic depicting the deletion of the predicted 7 nt seed region for the miR-29a binding site. (F) Luciferase assays were performed after transfection with either the full length or the 29a seed Del PMP22 3′UTR construct in the presence of miR-29a (*p<0.05) (n=3).