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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Glia. 2009 Sep;57(12):1265–1279. doi: 10.1002/glia.20846

Figure 6. Endogenous PMP22 RNA associates with Ago2 and the interaction is enhanced by miR-29a.

Figure 6

(A) C-myc-Ago2 transfected and non-transfected Schwann cells were processed for immunoprecipitation (IP) using either a non-specific rabbit IgG (Rbt IgG) or rabbit anti-c-myc conjugated (c-myc IP) agarose beads. Western blot using an anti-c-myc antibody is shown. The lack of signal in the non-transfected cells confirms the specificity of the IP. (B) RNA was isolated from total cell lysates (input), IP fractions (IP) and post-immunoprecipitation supernatants (Sup), from the samples analyzed in panel A. Semi-quantitative RT-PCR was performed using primers specific for PMP22 RNA. GAPDH was used as a control for equal total RNA input (*p<0.05; ***p<0.001; n=4).