Figure 8. PMP22 and miR-29 expression are inversely correlated in the developing rat sciatic nerve.

(A) Total RNA was isolated from rat sciatic nerves at the indicated time points and the expression of PMP22 was quantified by RT-PCR. GAPDH was used to normalize for loading and relative change in expression was determined using the 2^−ΔΔCT method (n=3, *p<0.05, ***p<0.001). (B) RT-PCR on the same RNA samples determines the expression of miR-29a in sciatic nerves during development. MiR-24 was used to normalize for equal loading (*p<0.05, ***p<0.001; n=3). (C) Data from the relative expression of PMP22 (x-axis) and miR-29a (y-axis), as determined in panels A and B, were analyzed by linear regression (p<0.05, r² = 0.078).