Skip to main content
. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Glia. 2009 Sep;57(12):1265–1279. doi: 10.1002/glia.20846

Figure 9. Nerve crush injury reduces PMP22 expression and elevates miR-29 levels.

Figure 9

(A) Total RNA was isolated from control or crush injured mouse sciatic nerves and relative expression of PMP22 was determined using quantitative RT-PCR. GAPDH was used to normalize for RNA loading (n=10 nerves, triplicates used for each run; ***p<0.001). Data are expressed as relative units. (B) Western blot analysis of total nerve lysates (5 μg/lane) at 5 d post-crush injury. GAPDH is shown as a loading control. Molecular mass, in kDa. (C) Relative quantitative RT-PCR was used to determine the levels of miR-29b at 4 d post-injury in mouse nerves. MiR-24 was used to normalize for equal loading (n=3 for each run and n=10 nerves for each condition, *p<0.05).