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. 2009 Aug;11(8):753–762. doi: 10.1593/neo.09466

Figure 1.

Figure 1

Generation of compound transgenic animals. (A) Breeding scheme used to generate matched sibling cohorts of Mdm2 haploinsufficient and Mdm2 wild-type mice with the homozygous human MYCN transgene in Svj-129 mice. The haploinsufficient mice were obtained from Dr. Lozano (MD Anderson Cancer Center) and backcrossed a minimum of seven generations into Svj-129 mice and then bred into the pTH-MYCN transgenic strain to obtain both Mdm2+/- and Mdm2+/+/MYCN+/+ compound transgenic mice. (B) Hematoxylin/eosin staining of both Mdm2+/- and Mdm2+/+ tumors shows an aggressive undifferentiated histologic finding. (C) Tumors in both Mdm2+/- and Mdm2+/+ were highly proliferative, and no differences in Ki-67 staining could be detected.