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. 1984 Sep;20(3):413–416. doi: 10.1128/jcm.20.3.413-416.1984

Comparison of lysis-centrifugation with a biphasic blood culture medium for the recovery of aerobic and facultatively anaerobic bacteria.

N K Henry, C M Grewell, P E Van Grevenhof, D M Ilstrup, J A Washington 2nd
PMCID: PMC271341  PMID: 6386858

Abstract

The Du Pont Isolator tube and Roche Septi-Chek blood culture bottle employ solid media which facilitate the removal of bacteria from static or cidal substances in blood to increase recovery and decrease detection time. In a comparison of 11,567 blood culture sets, the Isolator tube and vented Roche Septi-Chek bottle were positive for 533 (80%) and 494 (74%) of the aerobic and facultatively anaerobic organisms recovered, respectively. This difference was not significant. A significant difference was found in the overall detection time. The Isolator tube recovered the bacteria ca. 1 day earlier. The earlier detection time was most notable with Staphylococcus aureus, viridans streptococci, and Pseudomonas aeruginosa. Among the 355 bacteremic episodes analyzed by a computer program, the Isolator tube was responsible more often for the first report of bacteremia in a given patient. Both systems performed well for the recovery of aerobic and facultatively anaerobic bacteria, but it is recommended that either be used in combination with an unvented broth-containing bottle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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