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. Author manuscript; available in PMC: 2009 Jul 21.
Published in final edited form as: Nat Cell Biol. 2002 Jun;4(6):439–444. doi: 10.1038/ncb798

Figure 3. An unblocked Rpr N terminus is required for IAP binding and stabilization.

Figure 3

a, HEK 293T cells were transfected with Rpr, GST alone or GST—Rpr and FLAG-tagged XIAP protein. Proteins were precipitated with anti-FLAG or anti-Rpr antibodies and blotted with anti-FLAG. Proteins were precipitated with glutathione—Sepharose or anti-FLAG, and detected by immunoblotting with anti-GST or anti-FLAG. Although GST—Rpr and XIAP failed to coprecipitate, the untagged Rpr control clearly coprecipitated with XIAP protein. b, A pulse-chase experiment was performed as in Fig. 1c, using either wild-type Rpr (Rpr) or untagged Rpr lacking its first 15 amino acids (Rpr16-–65). The results were then quantified.