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. 2000 Sep 19;97(20):10984–10989. doi: 10.1073/pnas.190167297

Figure 4.

Figure 4

Proviral structure, integration, and expression in MSCVMLL-ELL mice. (A) Structure of the MSCVMLL-ELL retrovirus. Indicated are the diagnostic NheI, BamHI, and NcoI restriction endonuclease recognition sites that permit assessment of provirus integrity and copy number. PGK, phosphoglycerate kinase. (B) Southern blot analysis of NheI-digested genomic DNA with an MLL probe showing that leukemic spleens from MSCVMLL-ELL mice harbor nonrearranged (8.8-kb NheI band indicated by arrow) proviruses. The 10-kb fragment is representative of the endogenous MLL gene. At left is sizes (in kb) of the molecular weight standards. (C) Expression by reverse transcription–PCR of retroviral-derived MLL-ELL. Expression was studied in a negative control cell line (32D), MLL-ELL-transduced BM, a cell line derived from the leukemic BM of mouse #4 as well as in spleen from leukemic mice (spleen from mice #1 and #4 are shown), or from a control normal mouse.