TABLE 1.
WT KatG | KatG[S315G] | |
---|---|---|
Catalase activity (IU/mg) | 3800 ± 344 | 2039 ± 461 |
Peroxidase activity (IU/mg) | 0.9 ± 0.1 | 0.6 ± 0.15 |
CmpdI formation (PAA) (m−1s−1) | 3 × 104 | 7.4 × 104 |
CmpdI decay (PAA) (s) | ∼80 s | ∼40 s |
INH affinity (optical) (μm) | 1.0 | 0.7 |
INH affinity (ITC) (μm) | 1.6 | 1.4 |
Decay of 5-c heme (aging) (weeks) | ∼3 | ∼1 |