FIGURE 6.

Detection of VHH binding to BoNT LC protease. a, specific binding of the VHHs against A-LC and B-LC was confirmed in YS2H by Myc expression. b, New VHHs against B-LC protease were isolated by YS2H. The numbers in each plot indicate means ± S.E. of the MFI of the filled histograms from three independent measurements. c and d, SPR measurements of B8 (c) and G6 (d) binding to BoNT/A-LC. A-LC was injected at a series of 2-fold dilutions beginning at 160 nm to the B8-coated and 400 nm to the G6-coated chip. e, cysteines are highlighted in yellow for the pair that forms a conserved disulfide bond or in orange that forms extra disulfide bonds. The framework region (FR) and complementarity determining regions (CDR) are noted.