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. 2009 Apr 21;284(24):16135–16145. doi: 10.1074/jbc.M109.007484

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — OTUB1 interacts with ERα but not with PR-B. HEK293T cells were transfected with FLAG-ERα and HA-OTUB1 or empty vector controls. A, ERα was immunoprecipitated and analyzed by SDS-PAGE using antibodies against the HA epitope tag. B, reciprocal coimmunoprecipitation. HA-OTUB1 was immunoprecipitated and analyzed using indicated antibodies. C, ERα interacts with OTUB1 irrespective of E2. FLAG-ERα and HA-OTUB1 were expressed in the presence of 1 nm estradiol (E2) or vehicle and analyzed with indicated antibodies. D, OTUB1 does not bind to PR-B. FLAG-PR-B and HA-OTUB1 were transfected in HEK293T cells as previously described for ERα. PR-B was immunoprecipitated and analyzed for OTUB1 binding using the indicated antibodies. IB, immunoblot.