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. 2009 Apr 21;284(24):16135–16145. doi: 10.1074/jbc.M109.007484

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — OTUB1 C/S represses ERα-dependent transcription, whereas OTUB1 Ubmut rescues ERα mediated transcription. A, OTUB1 C/S represses ERα-dependent transcription. HeLa cells were transfected with empty vector, OTUB1, or OTUB1 C/S in the presence of ethanol or E2. The inset contains Western blot showing equal expression of OTUB1 and OTUB1 C/S in this experiment. B, OTUB1 Ubmut rescues ERα-mediated transcription. HEK293T cells were transfected with an ERE-luciferase reporter construct, ERα, OTUB1, and OTUB1 Ubmut or vector controls. ERα transcriptional activity was analyzed in the presence of E2 or ethanol vehicle. The inset contains a Western blot showing equal expression of OTUB1 and OTUB1 Ubmut in this experiment. The lower panel indicates E2-dependent ERα fold induction of transcription. IB, immunoblot.