FIGURE 5.

Effects of PhLP1 knockdown on the specificity of Gβ2 dimerization with Gγ subfamilies. A, the phylogenetic relationship between human Gγ subunits and RGS7 and 9 is depicted. An unrooted dendrogram was made using TreeView from a Gγ family sequence alignment created with ClustalX. The Gγ family can be separated into five subfamilies as indicated. The scale bar represents a substitution rate of 0.1 per amino acid. B, the Gγ/Gβ2 ratios within each Gγ subfamily under the different siRNA conditions from Fig. 3B were averaged and plotted to show the effects of PhLP1 knockdown on the subfamily specificity of Gβ2Gγ dimer formation. Error bars represent the S.E. of the mean within each subfamily. C, a similar average of the Gβ2Gγ ratios for each subfamily under the different PhLP1 overexpression conditions from Fig. 4B was calculated and plotted.