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. 2009 Apr 14;284(24):16400–16408. doi: 10.1074/jbc.M901201200

FIGURE 2.

FIGURE 2.

Isoform specificity of mRNA knockdown using a subunit isoform-specific siRNAs. siRNA pools from Dharmacon were used to reduce the mRNA levels of each isoform in MB231 cells as described under “Experimental Procedures.” The level of knockdown was quantitated by performing QRT-PCR on RNA from cells harvested 96 h siRNA post-treatment. Knockdown is reported as the ratio of the nanograms of mRNA in treated cells versus the nanograms of mRNA in nontreated (NT) cells as determined using the standard curve after QRT-PCR. Values are means; error bars indicate standard deviation, n = 5. *, p < 0.01 versus nontreated.