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. 2009 Apr 14;284(24):16409–16418. doi: 10.1074/jbc.M109.005736

FIGURE 4.

FIGURE 4.

NPM suppresses TPA-induced apoptosis. A, JB6 cells were transfected with either pcDNA3.1 or pcDNA3.1/NPM expression vector. a, after treatment of cells with TPA for 24 h, a TUNEL assay was performed, and the TUNEL-positive cells were counted and compared with DMSO control in their randomly selected fields. Data presented are relative -fold changes of TUNEL-positive cells compared with the pcDNA3.1-transfected DMSO control group (10–12% TUNEL-positive cells were observed in the control group). b, caspase 3 activity was determined in cellular extract following treatment with TPA for 1 h. B, HME cells were transfected with either pcDNA3.1 or pcDNA3.1/NPM expression vector and treated with TPA. a, mitochondrial p53 was detected 1 h after TPA treatment by Western blotting. b, -fold changes of TUNEL-positive cells were determined 24 h following TPA treatment. c, overexpression of NPM was confirmed by detecting the ectopic level of NPM. Each data point represents the mean ± S.D. of three experiments. **, p < 0.01 compared with pcDNA3.1-DMSO group; #, p < 0.01 compared with pcDNA3.1-TPA group. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.