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Effect of salt stress or hyperosmotic stress on the expression of NhaS3. A, wild type cells cultured in liquid BG11 medium (OD730 = 0.5) were transferred to the same medium (white bars) or to hyperosmotic medium containing either 500 mm NaCl (black bars) or 500 mm sorbitol (hatched bars) and cultured for the time indicated. B, cells samples taken from the cultures in A at the times indicated were subjected to Western blot analysis using anti-NhaS3 antibodies.
