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. 2009 Apr 22;284(24):16562–16574. doi: 10.1074/jbc.M109.010918

FIGURE 5.

FIGURE 5.

Confirmation of in situ phosphorylation of Kv1.2 at Ser-449. A, MS/MS spectrum resulting from isolation and collision-induced fragmentation of the singly charged m/z 773.4 precursor ion obtained from ion trap full scan MS spectrum of control HEK 293 cell Kv1.2-WT-myc tryptic digest. The sequence of the m/z 773.4 ion shown is based on a y-ion series (separated by vertical lines). B, MS/MS spectrum of the singly charged m/z 773.4 precursor ion obtained from ion trap full scan MS spectrum of 8-Br-cAMP-treated HEK 293 cell Kv1.2-WT-myc tryptic digest. The sequence is as indicated in A. amu, atomic mass units.