TABLE 2.
Comparison of the biophysical properties of Kv1.2-WT and Kv1.2-S449A
Parentheses indicate the number of cells. Current density and activation time constants were measured from whole-cell currents elicited by a step depolarization from −60 to +20 mV. Deactivation time constants were measured from tail currents elicited by a step to −50 mV from +20 mV. Current density, time constants, andV0.5for activation of Kv1.2-WT and -S449A were not significantly different (p> 0.05) by unpaired Student'st test.
| Current density at +20 mV | Activation time constant |
Deactivation time constant |
Activation V0.5 | |||
|---|---|---|---|---|---|---|
| τ1 | τ2 | τ1 | τ2 | |||
| pA/pF | ms | ms | ms | ms | ms | |
| Kv1.2-WT | 212.9 ± 50.2 (15) | 0.29 ± 0.05 (6) | 0.90 ± 0.17 (6) | 10.0 ± 1.6 (10) | 34.2 ± 5.7 (10) | −16.4 ± 3.9 (5) |
| Kv1.2-S449A | 187.5 ± 30.9 (11) | 0.5 ± 0.1 (6) | 1.5 ± 0.5 (6) | 9.6 ± 1.4 (9) | 33.5 ± 5.1 (9) | −14.4 ± 2.0 (4) |