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. 2009 Apr 20;284(24):16679–16692. doi: 10.1074/jbc.M109.009654

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Alignment and expression of zebrafish (D. rerio) Eaf1 and Eaf2/U19. A, zebrafish eaf1 (zEAF1; GenBank^TM accession number AAI53593) codes for a 253-amino acid protein with orthologues in X. laevis (xEAF1; GenBank^TM accession number AAI25802), Mus musculas (mEAF1; GenBank^TM accession number AAH41329), and Homo sapiens (hEAF1; GenBank^TM accession number AAH79658). B, zebrafish eaf2/u19 (zEAF2; GenBank^TM accession number CAQ15588) codes for a 259-amino acid protein with orthologues in X. laevis (xEAF2; GenBank^TM accession number CAE22450), M. musculas (mEAF2; GenBank^TM accession number AAH04721), and H. sapiens (hEAF2; GenBank^TM accession number AAO63811). C, whole mount in situ hybridization analysis of zebrafish eaf1 expression. D, whole mount in situ hybridization analysis of zebrafish eaf2/u19 expression. C1 and D1, blastula stage, lateral view; C2 and D2, shield stage embryos, lateral view; C3 and D3, 75% epiboly, lateral view; C4 and D4, 20 hpf, lateral view with anterior to the left; C5 and D5, 24 hpf, lateral view with anterior to the left; C6 and D6, 24 hpf, dorsal view with anterior on top; C7 and D7, 48 hpf, lateral view with anterior to the left; C8 and D8, 72 hpf, lateral view with anterior to the left; C9 and D9, 96 hpf, lateral view with anterior on top. b, brain; cg, cranial ganglion; fp, floor plate; ki, kidney; in, intestine; li, liver; sb, swim bladder; r, retina; ov, otic vesicle.