FIGURE 5.

Compound 3289-8625 blocks Wnt signaling. SuperTopflash 293 cells were treated with either 10 ng/ml (A) or 50 ng/ml (B) recombinant Wnt3a. The level of the Wnt pathway response according to luciferase activity was measured in the absence (gray bars) or presence (red bars) of 3289-8625 inhibitor at 3 μm. These data indicated that 3289-8625 could inhibit the Wnt pathway response at 10 ng/ml Wnt3a but that inhibition could be eliminated by increasing the level of Wnt pathway stimulation. Significance values are: in A, p = 0.05; in B, not significant. Error bars indicate S.E.