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Compound 3289-8625 inhibits Wnt pathway responses in culture and in vivo. A and B, control-treated (A) or inhibitor-treated (B) hyaloid vessels labeled with Hoechst 33258 (blue nuclei) and with the TUNEL technique for apoptotic cells (red nuclei). Quantification of the number of TUNEL-labeled cells (C) reveals that injections of 6 μm 3289-8625 reduced the level of cell death by about 2-fold. Significance values are: p = 0.0002. Error bars indicate S.E.
