TABLE 2.
List of synthesized PKG-specific substrate and inhibitor photoaffinity labels
| Synthesis |
Sequencea,b | Photolabel | Cross-link | |
|---|---|---|---|---|
| Peptide no. | Name | |||
| a-0 | W64-[Phe(Bz)-12] | TQAKRKKSLAMF*LR | Ph(Bz) | yes |
| b-1 | W64-[Phe(Tmd)-12] | TQAKRKKSLAMF*LR | Ph(Tmd) | yes |
| b-2a | W45-[Phe(Tmd)-8] | LRKKKKKF* | Ph(Tmd) | no |
| b-2b | DT-2-[Phe(Tmd)-21] | YGRKKRRQRRRPPLRKKKKKF* | Ph(Tmd) | no |
| b-2c | DT-2-[Phe(Tmd)-1] | F*GRKKRRQRRRPPLRKKKKKF* | Ph(Tmd) | no |
| b-3 | DT-2-[Phe(Tmd)-1,21] | F*GRKKRRQRRRPPLRKKKKKH | Ph(Tmd) | no |
| b-4a | W45-[Phe(Tmd)-8] | LRKKKKKF* | Ph(Tmd) | no |
| b-4b | DT-2-[Phe(Tmd)-21] | YGRKKRRQRRRPPLRKKKKKF* | Ph(Tmd) | no |
| b-4c | DT-2-[Phe(Tmd)-1] | F*GRKKRRQRRRPPLRKKKKKF* | Ph(Tmd) | no |
| b-5 | DT-2-[Phe(Tmd)-1,21] | F*GRKKRRQRRRPPLRKKKKKH | Ph(Tmd) | no |
| c-1a | W45-[Phe(Bz)-1] | F*RKKKKKY | Ph(Bz) | yes |
| c-1b | W45-[Phe(Bz)-2] | LF*RKKKKKY | Ph(Bz) | yes |
| c-2 | W45-[Phe(Bz)-6] | LRKKKF*KY | Ph(Bz) | yes |
| d-3a | DT-6-[Phe(Bz)-6] | YGRKKF*RQRRRPP | Ph(Bz) | yes |
| d-3b | DT-2-[Phe(Bz)-6,14] | YGRKKF*RQRRRPPF*RKKKKKY | Ph(Bz) | yes |
a Bold letter indicates the position of the isotopically labeled Leucine (L) or Arginine (R) or the position of the photoactivatable phenylalanine derivative (F*).
b Peptides are N-terminally acetylated and C-terminally amidated.