TABLE 3.

K_m and k_catvalues for the phos pho ryl a tion by PKA and PKG of synthetic peptides derived from previous studies

Peptidea	PKG			PKA			Index
	Km	kcat	kcat/Km	Km	kcat	kcat/Km	PKG/PKA
	μm	μmol/min/mg		μm	μmol/min/mg
PKI(5–24):
TYADFIASGRTGRRNSIb	2.4	1.0	0.42	0.12	1.0	8.33	0.05
IASGRTGRRNSIb	2.7	0.74	0.27	0.18	1.4	7.78	0.03
IASGRTGRRNSIb	7.0	0.79	0.11	0.32	0.56	1.75	0.63
W64:
TQAKRKKSLAMFLRc	0.26	11.5	44.23	1.7	3.9	2.29	19.2
TQAKRKKSLAMFc	0.5	11.0	22	3.3	5.7	1.72	12.9
TQAKRKKSLAc	1.74	11.1	6.37	2.68	7.9	5.22	2.16
RKISASEFDRPLRd	68	11	0.162	320	3.2	0.010	16.2
RKISASEFd	120	5.2	0.043	480	2.6	0.006	8.1
RKISASEd	80	5.6	0.070	93	3.7	0.040	1.8

^a The phosphate-accepting residue Ser is depicted in bold.

^b Taken from Mitchellet al.(13).

^c Taken from Dostmannet al.(11).

^d Taken from Colbranet al.(10).