TABLE 2.

ATP hydrolysis activity of engineered F₁F₀

Strain/Plasmid(s)	Gene producta	ATP hydrolysisb		Fold induction
		−LDAO	+LDAOc
KM2/pTAM37/pTAM46	bhis/bV5	0.30 ± 0.02	1.2 ± 0.06	4.0
KM2/pBR322	Δb	0.07 ± 0.01	0.2 ± 0.01	2.3
KM2/pSBC127	bV5	0.29 ± 0.01	1.3 ± 0.08	4.4
KM2/pSBC128	bI76C, V5	0.19 ± 0.01	0.9 ± 0.03	4.6
KM2/pSBC129	bR83C, V5	0.28 ± 0.07	1.2 ± 0.07	4.3
KM2/pSBC130	bA90C, V5	0.29 ± 0.04	1.2 ± 0.11	4.0
KM2/pSBC131	bE97C, V5	0.33 ± 0.07	1.1 ± 0.07	3.4
KM2/pSBC97	Tb	0.30 ± 0.02	1.0 ± 0.15	3.4
KM2/pSBC123	TbA83C	0.19 ± 0.03	0.7 ± 0.05	3.7
KM2/pSBC124	TbA90C	0.20 ± 0.04	0.7 ± 0.06	3.5
KM2/pSBC98	Tb′	0.17 ± 0.02	0.5 ± 0.06	2.9
KM2/pSBC125	Tb′A83C	0.10 ± 0.01	0.2 ± 0.01	2.2
KM2/pSBC126	Tb′A90C	0.10 ± 0.01	0.2 ± 0.01	2.3
KM2/pSBC97/pSBC98	Tb/Tb′	0.24 ± 0.02	0.9 ± 0.04	3.7
KM2/pSBC123/pSBC125	TbA83C/Tb′A83C	0.19 ± 0.01	0.6 ± 0.01	3.2
KM2/pSBC123/pSBC126	TbA83C/Tb′A90C	0.23 ± 0.02	0.8 ± 0.02	3.4
KM2/pSBC124/pSBC125	TbA90C/Tb′A83C	0.19 ± 0.02	0.7 ± 0.05	3.8
KM2/pSBC124/pSBC126	TbA90C/Tb′A90C	0.21 ± 0.02	0.8 ± 0.07	3.6

^a See Table 1 for gene product definitions.

^b Reported in μmol of P_i/mg of membrane protein/min.

^c Used to release F₁from the inhibitory effect of F₀.