FIGURE 2.

SEC of detergent-solubilized cross-linked colicin Ia. A, elution profiles of cross-linked colicin Ia in proteoliposomes after solubilization with 2% DDM and using Superose 6 column. B, calibration curve using the retention volumes of standard proteins with known Stokes radii. C, 0.5-ml fractions for the elution profiles in A precipitated with trichloroacetic acid and resolved on a 3–8% Tris acetate polyacrylamide gel; lanes 1–7 correspond to the range 9.5–13 ml; lanes 8 and 9 correspond to 13.5–14.5 ml. Species with a large molecular mass are retained in the stacking portion of the gel particularly in lanes 8 and 9. BSA, bovine serum albumin; AU, absorbance units.