FIGURE 7.
Inhibition of p66ShcA attenuates NL4-3-induced phosphorylation of Foxo3A at Thr-32. Panel A, protein extracts were isolated by PAGE, and nitrocellulose filters were probed with phospho-antibodies to Ser-36 of p66ShcA, Ser-473 of Akt/PKB, and Thr-32 of Foxo3A. Conversely, as shown in Panel B, mu-36 had no detectable effect on Foxo3A regulatory residues Ser-253 or Ser-318/321. Panel C, infection of human PBMC by HIV-1HT/92/599 enhances phosphorylation at Ser-36 of p66ShcA protein. PBMC were incubated in SFM containing HIV-1HT/92/599 or buffer (C) for 6 and 24 h. PBMC were lysed and prepared for immunoblot analyses using anti-ShcA/p66 (Ser(P)-36) antibody.