Figure 2.

Characterization of membrane anchored recombinant forms of IRAC I and IRAC II. CRFK cells were transfected with pcDNA™4/TO derived vectors encoding the different membrane anchored recombinant forms of IRAC. After 18 h, cells were fixed and permeabilized (panels (i) to (iii) and (vii) to (ix)) or only fixed (panels (iv) to (vi) and (x) to (xii)), then treated as described in the methods for indirect immunofluorescent labeling. mAbs anti-IRAC I or II were used as primary antibodies and were revealed by Alexa488-GAM secondary antibody. The side of each panel corresponds to 30 μm of the specimen. (For a color version of this figure, please consult www.vetres.org.)