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. 2009 Jun 18;40(5):51. doi: 10.1051/vetres/2009034

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© INRA, EDP Sciences, 2009

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any noncommercial medium, provided the original work is properly cited.

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Figure 4. — Membrane anchored forms of IRAC proteins are detectable at the surface of PBMC infected ex vivo. Rabbit PBMC were collected as described in the methods and infected at a MOI of 1 PFU/cell with BoHV-4 V. test BAC G IRAC I CD46, V. test BAC G IRAC I HA, V. test BAC G IRAC II CD46 or V. test BAC G IRAC II HA strains. After 48 h, cells were treated as described in the methods for indirect immunofluorescent labeling with mAbs directed against rabbit CD11b, rabbit IgMs (α-B) or rabbit CD5 (α-T) and then analyzed by flow cytometry for eGFP and Alexa-633 fluorescences.