Figure 2.

ELISA of anti-C2 antibodies produced at week 6 (A) and 11 (B) by rabbits immunised with C2 alone, or in combination with different adjuvants. C2 was coated onto ELISA plates (1 μg/mL in 50 mM carbonate buffer, pH 6, 16 h, 4 °C) and probed for recognition by IgG collected at weeks 6 and 11 from rabbit A1 (○; solid line), A2 (○; broken line), B1 (▲; solid line), B2 (▲; broken line), F1 (×; solid line), F2 (×; broken line), R1 (■; solid line), and R2 (■; broken line), and non-immune IgG (♦). Absorbance readings at 405 nm represent the average of two experiments. Goat anti-rabbit IgG-HRPO labelled antibodies were used for detection and ABTS/H₂O₂ as substrate as described in Materials and methods.