(A) Coomassie blue staining of GST fusion
proteins. GST alone, GST-ICP0 20–241, GST-ICP0 543–768, and
UL34 1–240 were purified with the aid of
glutathione-agarose beads, electrophoretically separated on 10%
bisacrylamide gels, and stained with Coomassie blue.
(B) Autoradiographic image of cdc2-mediated
phosphorylation of histone H1 or indicated GST fusion proteins. Cdc2
was immunoprecipitated from uninfected HeLa cells with cdc2 antibody,
and histone H1 or GST fusion proteins were tested as substrates for
cdc2. Reactions were electrophoretically separated on 10%
bisacrylamide gels, transferred to nitrocellulose membrane, and
exposed to film.