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. 2009 Jun 9;101(1):38–47. doi: 10.1038/sj.bjc.6605101

Figure 3.

Figure 3

Effects of combination treatment on cell cycle progression and phospho-Src. (A) Cells were treated 48 h with either drug alone or in combination (1 : 1 ratio), then collected and assessed for cell cycle distribution by 7-AAD staining. Flow cytometry was used to analyse staining of fixed cells. (B) Protein lysates of MDA-MB-231, MCF7, and T47D cells treated for 2 h were immunoblotted as indicated for pSrc-Y416, stripped and reprobed for total Src. (C) Cells were treated for 48 h with DMSO, 100 nM dasatinib, 100 nM doxorubicin, or 100 nM of each drug. Whole lysates were immunoblotted for PARP, p21WAF1, and GAPDH.