Table 2. Antibodies used for the immunohistochemical study.
| Antibody | Clone | Source | Dilution | Antigen retrieval |
|---|---|---|---|---|
| Ki67 | Monoclonal MIB-1 | DakoCytomation, Glostrup, Denmark | 1 : 50 | Treatment in hot bath (95–98°C) for 40 min (Dako Target Retrieval Solution (pH 9.0)) |
| Mcm3 | Monoclonal 3A2 | MBL, Nagoya, Japan | 1 : 400 | Microwave treatment for 15 min (Dako Target Retrieval Solution (pH 9.0)) |
| Bax | Monoclonal B-9 | Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA | 1 : 100 | Microwave treatment for 15 min (citrate buffer (pH 6.0, 0.01 mol/l)) |
| Bcl-2 | Monoclonal 124 | DakoCytomation | 1 : 50 | Microwave treatment for 15 min (Dako Target Retrieval Solution (pH 9.0)) |
| ssDNA | Polyclonal A4506 | DakoCytomation | 1 : 400 | Not applied |
| Grp78 | Polyclonal H-129 | Santa Cruz Biotechnology, Inc. | 1 : 100 | Microwave treatment for 30 min (citrate buffer (pH 6.0, 0.01 mol/l)) |
| TS | Monoclonal TS106 | DakoCytomation | 1 : 50 | Autoclave (121°C) treatment for 15 min (citrate buffer (pH 6.0, 0.01 mol/l)) |
| DPD | Polyclonal RDPDPA | Taiho Pharmaceuticals Co., Ltd., Tokyo, Japan | 1 : 400 | Autoclave (121°C) treatment for 10 min (EDTA (pH 8.0, 1 mmol/l)) |
| CD34 | Monoclonal QBEnd 10 | DakoCytomation | 1 : 500 | Not applied |
| VEGF | Polyclonal A-20 | Santa Cruz Biotechnology, Inc. | 1 : 100 | Not applied |
| Nestin | Polyclonal N1602 | IBL, Takasaki, Japan | 1 : 500 | Not applied |
| LAT1 | Monoclonal | Fuji Biomedix, Tokyo, Japan | Prediluted | Microwave treatment for 5 min (in the buffer supplied by kit) |
Mcm, minichromosome maintenance; Grp78, glucose-regulated protein 78; TS, thymidylate synthase; DPD, dihydropyrimidine dehydrogenase; VEGF, vascular endothelial growth factor; LAT1, L-type amino-acid transporter 1.