Skip to main content
. Author manuscript; available in PMC: 2009 Jul 21.
Published in final edited form as: Cancer Res. 2008 Jun 1;68(11):4229–4238. doi: 10.1158/0008-5472.CAN-07-5272

Figure 1.

Figure 1

Morphology and multilineage differentiation potential of MSCs. Cultures of rhesus MSCs (A) and human MSCs (B) capable of proliferating for several passages and exhibiting fibroblastic morphology in P1. Osteogenic differentiation of MSCs was confirmed by staining with Alizarin red. Adipogenic differentiation was confirmed by staining with Oil Red O. Chondrogenic differentiation was detected with Toluidine Blue. C, senescence was assessed by counting the number of reactive cells to senescence-associated β-galactosidase in cultures of four MSC types at low and higher passages. D, mitotic indeces of MSCs in early and late passage cultures. Single-variable histograms of DNA content indicate the percentages of cells in G0-G1, S, and G2-M phases of the cell cycle. The data are obtained from staining the DNA of P1, P10, P20, and P30 cultures of MSC types. rBMSC and rASCs showed a polyploid fraction in passage 20; a greater fraction of polyploidy (~18%) was seen in rBMSCs P30. hBMSC and hASC showed increasing fraction of cells arrested in S phase in P10 to P30.