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. Author manuscript; available in PMC: 2009 Jul 21.
Published in final edited form as: Cancer Res. 2008 Jun 1;68(11):4229–4238. doi: 10.1158/0008-5472.CAN-07-5272

Figure 4.

Figure 4

The effect of prolonged in vitro culture on MSC apoptosis. A, the expression of the p53 gene in low and high passage cultures of rASCs and hBMSCs. B, Western blot analysis of the expression level of p53 protein in MSCs. C, MSCs were examined for apoptosis using a fluorescent DNAfragmentation assay (TUNEL stain). Microscopic fields of MSCs from P1, P20, and P30 were photographed individually to detect apoptosis cells. D, apoptosis level in MSC populations were performed by FACS using TUNEL stain.