Fig. 10. NO production and expression of Foxp3 in wild-type, iNOS heterozygous and iNOS homozygous knockout mouse splenocytes.

Splenocytes isolated from wild-type (A-C), iNOS (+/−) (D-F), and iNOS(−/−) (G-I) knock-out mice were treated with different doses of p40₂. (A, D, G) After 24 h, supernatants were used for nitrite assay as described under ‘Materials and Methods’. Data are mean ± SD of three different experiments. ^ap < 0.001 vs control. Splenic T cells were isolated after 24 h, followed by mRNA analysis of Foxp3 by semi-quantitative RT-PCR (B, E, G) and real-time PCR (C, F, I). ^ap < 0.001 & ^bp < 0.05 vs control.