Figure 2.
ADP stimulation of the respiratory burst induces the dissociation of Trx from ASK1. NR8383 cells were stimulated with 400 µM ADP and ASK1 was immunoprecipitated. Catalase (100 U/ml) was added 15 min before stimulation. (A) ADP induces Trx dissociation from ASK1. (B). Catalase eliminated ADP-induced dissociation of Trx from ASK1. Experiments were repeated at least three times and the blots shown are representative of the results obtained. The bar graph (B) represents the mean and standard deviation of photon counts as described in Methods; n = 4, *p < 0.05.
