Fig. 2.

ROS accumulate when BMDMs or mDCs, but not gp91phox^−/− BMDMs, are infected with sod5Δ/Δ cells. A–C. ROS measurement by luminol-dependent chemiluminescence at 37°C in 2.5 min intervals over a 90 min period [relative luciferase units (RLU) min⁻¹ per 1000 immune cells]. A. (a) Stimulation of BMDMs with either the wild type (CA-IF100) strain or the sod1Δ/Δ (CA-IF003), sod4Δ/Δ (CA-IF015), sod5Δ/Δ (CA-IF019) mutant strains or sod5Δ/SOD5 heterozygous strain (CA-IF017) (MOI 5:1). (b) Stimulation of BMDMs with the sod5Δ/Δ::SOD5 revertant (CA-IF027) (MOI 5:1) or PMA (10 nM). (c) Quantification of the total ROS release between 10 and 60 min (striped area) by calculating the area under the curve (MOI 5:1). The average of three independent experiments is presented. *Infection with sod5Δ/Δ yields 4.3 ± 0.68 times more ROS than with wild-type C. albicans.**P < 0.02. B. (a) Stimulation of mDCs with either the wild type (CA-IF100) strain, or the sod1Δ/Δ (CA-IF003), sod4Δ/Δ (CA-IF015), sod6Δ/Δ (CA-IF023) or sod5Δ/Δ mutant strains. (b) Quantification of the total ROS release between 10 and 60 min (striped area) by calculating the area under the curve. The average of three independent experiments is presented. *Infection with sod5Δ/Δ yields 4 ± 0.64 times more ROS than with wild type cells. **P < 0.05. C. Stimulation of gp91phox^−/− or wild-type BMDMs with either the wild-type (CA-IF100) strain, the sod5Δ/Δ (CA-IF019) mutant strain or sod5Δ/Δ::SOD5 re-integrant (CA-IF027). A–C. Results of one experiment per condition are shown. Data were reproduced in at least three independent experiments. Statistical significances were calculated using a two-tailed Student's t-test.