Table 2.

Quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of PYD expression in wild-type and pyd mutants

	Relative expression (E−ΔCt)
Sample	PYD1	PYD2	PYD3
Wild-type	0.150 ± 0.0084	0.070 ± 0.0044	0.037 ± 0.0022
pyd1-1	0.003 ± 0.0004**	0.079 ± 0.0146	0.044 ± 0.0070
pyd1-2	0.003 ± 0.0012**	0.076 ± 0.0047	0.057 ± 0.0153
pyd2-1	0.103 ± 0.0084*	0.008 ± 0.0040**	0.023 ± 0.0013*
pyd2-2	0.103 ± 0.0195*	0.000 ± 0.0000**	0.023 ± 0.0031*
pyd3	0.080 ± 0.0039**	0.040 ± 0.0010**	0.001 ± 0.0001**

Values are expressed as the difference in the Ct value relative to that of elongation factor 1α(EF1α) taken to the power of efficiency. Each value represents the mean ± standard error for six individual seedlings. Measurements were repeated twice.

^*Significant differences (P < 0.05) using unpaired two-tailed t-tests.

^**Highly significant differences (P < 0.001).