Table 4.

Quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of PYD1 expression in congeneric wild-type and pyd1 mutants

	Relative expression (E−ΔCt)
Primer pair	PYD1	pyd1
PYD1 268–342	0.0102 ± 0.0010	0.0067 ± 0.0012
PYD1 530–590	0.0097 ± 0.0003	0.0057 ± 0.0001
PYD1 613–683	0.0110 ± 0.0007	0.0063 ± 0.0002
PYD1 1054–1143	0.0265 ± 0.0011	0.0003 ± 0.0002

Values are expressed as the difference in the Ct value relative to that of EF1αtaken to the power of efficiency. Each value represents the mean ± standard error for three individual seedlings. Measurements were repeated twice.