Figure 1.

Each of the prgs is required for type III secretion. SDS/PAGE analysis of secreted proteins from WT S. typhimurium (CS401), isogenic strains harboring nonpolar deletions of each of the prg genes; ΔH (TK164), ΔI (TK25), ΔJ (TK26), ΔK (TK93) and each of the corresponding complemented strains; ΔH + pWprgH (pTK30), ΔI + pWprgI (pTK31), ΔJ + pWprgJ (pTK32), and ΔK + pWprgK (pTK33). The samples were prepared from overnight cultures grown with mild aeration. The identity of proteins secreted by the SPI1 TTSS and flagellar system are indicated on the left.