Skip to main content
. 2000 Sep 12;97(20):11008–11013. doi: 10.1073/pnas.200209497

Figure 4.

Figure 4

Prg complex isolation from E. coli. (A) SDS/PAGE of whole cell lysates (WC) and needle complex fractions (NC) prepared from recombinant E. coli (DH5α) expressing prgH (pTK43), prgK (pTK44), or prgH/prgK (pTK53). (B) Western blot analysis of PrgH isolated from E. coli (from A). The sample was resuspended in SDS loading buffer and boiled (+) or directly loaded (−) onto the gel. Blot was probed with polyclonal antiserum to PrgH. (C and D) Electron micrographs of (C) PrgH isolated from E. coli, and (D) NC isolated from WT S. typhimurium. In both C and D, numerous tetrameric-shaped structures can be seen. (Scale bar, 50 nm.) (E) Enlarged image of one of the tetramers from C with a schematic showing an approximate size of 10 nm × 10 nm (n = 25). (Scale bar, 50 nm.)