Table 2.
Neutral amino acid concentrations in arterial plasma and brain pools
| Arterial plasma
acid-soluble pool, μM
|
Tissue total acid-soluble pool,
nmol/g
|
Tissue tRNA-bound pool,
pmol/g
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| BTBR (24) | HTZ (22) | HMZ (19) | BTBR (14) | HTZ (12) | HMZ (10) | BTBR (14) | HTZ (12) | HMZ (10) | |
| Valine | 213 ± 11 | 260 ± 16* | 253 ± 11 | 61 ± 2 | 58 ± 3 | 32 ± 3§‡ | 104 ± 10 | 108 ± 8 | 122 ± 11 |
| Methionine | 49 ± 3 | 60 ± 4 | 46 ± 4† | ND | ND | ND | 56 ± 4 | 55 ± 5 | 60 ± 9 |
| Isoleucine | 99 ± 4 | 115 ± 6 | 113 ± 4 | 31 ± 1 | 30 ± 1 | 21 ± 2§‡ | 98 ± 4 | 105 ± 4 | 113 ± 6 |
| Leucine | 176 ± 8 | 209 ± 13 | 184 ± 8 | 58 ± 1 | 57 ± 2 | 37 ± 3§‡ | 128 ± 4 | 138 ± 4 | 146 ± 7 |
| Tyrosine | 44 ± 3 | 52 ± 4 | ND | 37 ± 3 | 32 ± 2 | 12 ± 2§‡ | 38 ± 4 | 37 ± 5 | 46 ± 5 |
| Phenylalanine | 71 ± 3 | 98 ± 6‡ | 1513 ± 70‡§ | 57 ± 3 | 62 ± 4 | 472 ± 86¶∥ | 63 ± 4 | 63 ± 4 | 74 ± 6 |
Values are means ± SEM for number of mice indicated in parentheses. Plasma phenylalanine was determined in 17 HMZ mice, and tRNA-bound valine was quantified in 13, 11, and 9 BTBR, HTZ, and HMZ mice, respectively. ND, not determined. Statistically significantly different from BTBR, *P < 0.05; ¶, P < 0.01; ‡, P < 0.001. Statistically significantly different from HTZ, †, P < 0.05; ∥, P < 0.0;, §, P < 0.001.