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. 2009 Mar 30;587(Pt 11):2429–2442. doi: 10.1113/jphysiol.2009.172254

Figure 9. TRPC1 interacts with STIM1 to form SOCs in mouse PASMCs.

Figure 9

A, left panel, TRPC1 was detected in cultured mouse PASMCs in the absence and presence of store depletion. Right panel, bar graph showing expression levels of TRPC1 measured relative to GAPDH in control cells (denoted as 1, filled bar), and in cells subjected to store depletion (open bar). Data are means ±s.e.m. of 3 separate Western blot analyses. B, left panel, STIM1 was detected in cultured mouse PASMCs in the absence and presence of store depletion. Right panel, bar graph showing expression levels of STIM1 measured relative to GAPDH in control cells (denoted as 1, filled bar), and in cells subjected to store depletion (open bar). Data are means ±s.e.m. of 3 separate Western blot analyses. C, STIM1 co-immunoprecipitated TRPC1 in cultured mouse PASMCs in the absence and presence of store depletion. STIM1 was first immunoprecipitated (IP) with EXBIO STIM1 antibody (10 μg) and the blot was subsequently probed with BD Biosciences STIM1 antibody (WB, 1 : 100). The blot was then probed for co-IP of TRPC1 expression using TRPC1 antibody (WB, 1 : 100, Alomone). Experiments were performed in 3 separate co-IP procedures and Western blot analyses.