Figure 5. Effect of postnatal EO on expression of proteins of the leptin signalling pathway in in hypothalamus of adult rats (180 days).

Homogenates of hypothalamus from SL and NL groups were obtained, and detection of Ob-R (B; n= 7), JAK2 (C; n= 7), STAT3 (D; n= 7) and p-STAT3 (E; n= 7) was done by Western blotting. The bands in A represent the expression of proteins in hypothalamus homogenate from individual NL and SL animals. The Ob-R, JAK2, STAT3 and p-STAT3 contents were quantified by scanning densitometry of the bands. Actin was loaded as a control. Results are expressed as means +s.e.m.*P < 0.05. A representative experiment is shown from three independent experiments.